FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42131170
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG27250.015.356029526
ATAGGGG108600.012.24204738
GGCATAG105600.012.03629535
AGCTTAG108700.012.02372145
TGTCGTT38800.011.42242328
CATAGGG117450.010.93663337
GCTAAGT40350.010.93176814
GCATAGG120350.010.691854536
GGGAGGT153250.010.4700913
GAGCTTA125550.010.42792944
ATATAGC51050.010.1833586
GGAGGTC159250.09.9768954
AACTCTA100550.09.8214345
TGGCATA136250.09.59286734
TATAGCT52350.09.5436437
GTCGTTC47450.09.24532429
TTGTCGT52650.08.93045827
ACTCTCC189400.08.69781211
CACCGAA105950.08.68389745
GCCGACA106200.08.55752439
Produced by FastQC (version 0.11.5)