FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51237177
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1473800.28764270131432107No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG544810.10633099477748355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC281950.025.9102464
ACCGCGT283800.025.6224676
GACCGCG286150.025.3727385
CAGACCG294250.024.980043
CCGCGTT298150.024.4118827
CGCGTTC313900.023.244138
TCAGACC332350.022.5634752
GAACGAT327350.022.06149345
AATACGG345150.020.82074732
AGAACGA348800.020.7025544
GCGTTCT358000.020.4750589
ATACGGA353700.020.29903233
TACGGAG353250.020.2170134
CTCAGAC430000.019.385381
CAATACG377700.019.16951431
ACGGAGA381150.018.78461535
AAGAACG388500.018.62187243
TCGGCGC95200.018.52714745
TTCGGCG98300.018.20453344
CCCAATA404200.018.13324229
Produced by FastQC (version 0.11.5)