FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51237177
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA587010.11456720185813517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG163150.016.3220145
GGCATAG174650.015.36583935
GGTCACT272200.014.7789227
ATAGGGG186700.014.49285838
GAGCTTA186300.014.48726244
GTCACTC286550.014.1318748
ACTCTCC288200.013.791429511
TGGCATA199450.013.65923434
AGGTCAC297400.013.6478846
CATAGGG199800.013.47544937
GGGAGGT325800.013.325233
GGAGGTC307750.013.2428364
GAGGTCA312650.013.1637135
CGTCGAT31800.012.7363312
AGGGATC150600.012.4249662
TAGCGTA11000.012.2688337
GCATAGG221950.012.17167336
TCACCCG103950.011.5024083
GCGTCGA35750.011.45797611
TCACTCT357400.011.4374789
Produced by FastQC (version 0.11.5)