FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51468362
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG581880.1130558613852914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGC51500.013.3588036
GGGAGGT325850.013.07488253
GTTGTCG48650.012.3967926
GGAGGTC335650.012.3419654
GTCACTC332650.012.2136028
GGTCACT327200.012.1419847
GAGGTCA342950.012.1120425
AGGTCAC339600.011.936626
TATAGCT57800.011.7864017
TGTCGTT50500.011.54199528
ACTCTCC363900.011.09280311
GCTAAGT57800.010.86401414
AGCTTAG274200.010.82861545
TGGGAGG307200.010.5020912
GGCATAG285050.010.453402535
TCACTCT403550.010.174219
CACTCTC401250.09.99872510
ATAGGGG299600.09.91954838
TAGCGTA10700.09.8831137
TCAGACG116550.09.8447722
Produced by FastQC (version 0.11.5)