FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49261846
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT701730.14244898577288395No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG579180.11757172071870794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT149550.025.104796
AGACCGC153200.024.5733154
CCGCGTT159050.023.562867
GACCGCG160100.023.4579985
CGCGTTC163950.022.8997948
CAGACCG168650.022.3623683
GAACGAT180850.020.13706245
TCAGACC205150.018.8339042
GCGTTCT203650.018.5461169
AATACGG201450.018.11357532
AACGCTA189000.017.91351323
TACGGAG201900.017.86104434
ATACGGA204500.017.82098633
ACGCTAC192350.017.5670124
AGAACGA208500.017.56528944
CGCTACC197250.017.0964425
TTCGACG15050.016.7404926
ACGGAGA222200.016.30012735
TCACGCT216700.016.297748
AAAACGC209650.016.21281621
Produced by FastQC (version 0.11.5)