FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49261846
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG28000.015.18605326
ACCCGGC80500.014.9605455
GGCATAG171300.014.41860135
AGCTTAG173400.014.29460945
GGAGGTC261950.014.0263344
ATAGGGG182700.013.76435338
TCACCCG90850.013.7286883
GGGAGGT313550.012.8382233
GAGCTTA198250.012.69624144
ACGGACA96100.012.66432318
TGGCATA199250.012.52041734
CCGGACA100150.012.24510712
CATAGGG204750.012.2163137
CACCCGG103300.012.0304324
CGGACAC103100.011.7634413
TGTCGTT37750.011.621716528
CGGACAG103000.011.48765719
GCATAGG218350.011.41433336
CACGGAC109450.011.03745217
CGGCCCG114200.010.9574288
Produced by FastQC (version 0.11.5)