FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68088215
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1057950.15537931197050767No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA956730.14051330321994784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC129000.024.8721854
GACCGCG147400.021.8742315
CAGACCG148150.021.7183953
ACCGCGT153000.021.1765026
CCGCGTT270550.018.4839971
AACGCTA166200.017.3003923
ACGCTAC167000.017.17709724
TGCGCGT70350.016.75828745
CGCTATG72950.016.62332729
ATGCGCG71250.016.51513544
CGCTACC176200.016.33129925
CGACGTC75450.016.16203723
GTCGCTA75800.015.96862527
GAAAACG165750.015.90856720
TTCGGCG137200.015.77561344
TCAGACC220650.015.6340722
CGCGTTC313350.015.5834612
TCGCTAT78000.015.51822828
TCGGCGC140950.015.35579945
CCCGTAT41050.014.664931
Produced by FastQC (version 0.11.5)