FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68088215
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1137820.1671096826374432No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG878860.1290766691416422No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC747510.10978551868337273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG194450.017.39780845
GGCATAG199850.016.91613235
ACCCGGC169900.016.7261095
CATAGGG215350.015.70746537
TCACCCG192450.015.6646353
GAGCTTA222100.015.40362144
GTTGTCG38900.015.19669526
GTCACTC328950.014.867318
GGTCACT329300.014.6126617
ATAGGGG237100.014.57218438
AGGGATC257700.014.3744942
CGGACAC198350.014.32744913
TGGCATA238950.014.24216134
CCGGACA202450.014.225903512
GCATAGG241350.014.00741236
ATAGCTC199250.013.75856139
ACTCTCC361450.013.67984211
CGGACAG203400.013.66477219
TAGCATA147550.013.4744029
ACGGACA211100.013.37868518
Produced by FastQC (version 0.11.5)