FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44326747
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA80150.019.68036723
ACGCTAC82150.019.42067124
TTCGACG17800.018.7073126
CGCTACC86150.018.30999825
ACCGCGT75400.016.829746
AAAACGC96900.016.46470521
GACCGCG77600.016.3816975
AGACCGC79850.015.8637414
GTCCGTT36000.015.69094851
CCGCGTT81050.015.6834687
CGCGTTC86050.014.7971618
GAAAACG110500.014.51711420
TCGGCGC54100.014.42950545
CCCGTAT30000.014.4031661
CAGACCG90850.014.2154013
AAACGCT113650.014.03765322
GAACGAT89950.014.00574145
TCGACGA24400.013.8308257
TCACGCT126250.013.7918468
ACGTTCG15550.013.7455746
Produced by FastQC (version 0.11.5)