FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44326747
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG105200.012.68294545
GGCATAG106650.012.53169135
ATAGGGG115900.011.74522738
CATAGGG115950.011.39072737
ACCCGGC69800.011.02415855
GAGCTTA126250.010.80012444
GCATAGG123050.010.71519636
GTCACTC179550.010.5256658
GGGAGGT209300.010.2989193
GGTCACT184650.010.1373237
AGGTCAC200300.09.9406576
TGGCATA138950.09.79672534
GGAGGTC207050.09.7370224
ACTCTCC201950.09.45840811
GAGGTCA220650.09.2588425
ACGGACA80100.09.18532318
ATAGCTC75750.09.118649539
GTTGTCG24350.08.96276926
TCACCCG93750.08.7602593
CGTCGAT19050.08.73960812
Produced by FastQC (version 0.11.5)