FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42386531
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG26850.022.4574076
TCGACGA32450.018.9979867
CGGTATA47950.017.73674220
AACGCTA76700.017.07254423
ATGCGCG25700.017.07145744
CGACGAG35050.016.754228
ACGCTAC79200.016.59045624
GTCCGTT33500.016.1394861
TGCGCGT27350.015.87730445
CGCTACC82700.015.8338925
CCCGTAT27000.014.8518131
GTCGGTT38700.014.49478151
AAAACGC93600.014.278439521
GCACTAC56000.014.14245425
CCGTACG53400.013.7355314
CTCCGTT61800.013.7063751
CTCGGTT78450.013.4392831
TCACGCT108100.013.3622938
CTCCGGT57550.013.309351
CGTACGC53600.013.3064925
Produced by FastQC (version 0.11.5)