FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49101341
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG798690.16266154523152432No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG684940.13949517183247603No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA604320.1230760683297835No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG601990.12260153953840079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT374700.014.6682353
GGAGGTC406150.013.38785654
TGCCCGT259050.013.03527444
GTTGTCG32500.013.01650526
TGGGAGG353700.012.1243062
GGTCACT442150.012.0177137
GTCACTC442450.011.9842158
GAGGTCA460750.011.9524695
ACCCGGC85150.011.91525655
ACTCTCC449200.011.88926911
GCCCGTG290700.011.75169445
TCAGACG123300.011.7332122
AGGTCAC475950.011.603846
ATATAGC41400.011.5198566
AGGGATC162850.011.301462
TAGCGTA13000.011.25054537
CGGACAC91550.010.98410613
TCACCCG100500.010.8123263
AGCTTAG388100.010.48625545
CACTCTC512300.010.41608510
Produced by FastQC (version 0.11.5)