FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53036404
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT955380.1801366472734464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC200600.023.6081854
ACCGCGT204100.023.1923166
CAGACCG216200.021.9879723
CCGCGTT216900.021.782177
GACCGCG218850.021.5880855
GAACGAT217450.021.54252845
CGCGTTC223150.021.202348
TCAGACC254900.019.09982
AGAACGA246750.019.01145244
AATACGG251800.018.45271132
ATACGGA260500.017.95736533
GCGTTCT266200.017.9425149
TACGGAG262050.017.6106334
CTCAGAC330350.016.8266411
CAATACG280150.016.72194331
ACGGAGA284550.016.2102135
TCGGCGC80950.015.95403245
AACGCTA173400.015.73829223
AAGAACG298650.015.7380243
TTCGGCG84250.015.70268144
Produced by FastQC (version 0.11.5)