FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53036404
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG164200.015.22675335
GTTGTCG25550.015.05973826
AGCTTAG165550.015.02407445
ATAGGGG185700.013.74267438
TGGCATA187800.013.4570734
CATAGGG187450.013.37445237
GAGCTTA190450.013.31921744
ACCCGGC86050.013.1156865
GGAGGTC282050.012.9215244
GGGAGGT312850.012.8416243
GCATAGG198750.012.57985836
ACGGACA95750.011.72544418
TCACCCG102500.011.3551913
ACTCTCC322150.011.16217711
TAGGGGA320150.010.97232239
GGTCACT329100.010.699787
CGGACAC104200.010.69099813
CCGGACA105250.010.49847312
TCGCGCT41050.010.47308945
ATAGCTC103500.010.45812639
Produced by FastQC (version 0.11.5)