FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54409900
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1000370.1838580846500361No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG546970.10052766132633952No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA544440.10006267241807097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG70550.019.17083426
ATATAGC79950.018.600476
TATAGCT93550.016.1846247
GCTAAGT88050.016.17329614
TGTCGTT88300.015.54735928
GGGAGGT473600.015.4683933
GGAGGTC464750.015.4040644
CAATATA107500.015.0687964
AATATAG120550.014.4265225
AGCTTAG351050.014.37397845
GTCGTTC97500.013.84950129
TTGTCGT105300.013.635221527
TCGTTCC99150.013.59632630
TACAATA118550.013.5510312
CGTTCCC99700.013.45360231
GAGGTCA539550.013.4059825
ATAGGGG387750.013.23029538
TGGGAGG411600.013.113862
GGCATAG391200.013.05079435
GTCACTC540550.013.0265528
Produced by FastQC (version 0.11.5)