FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55094680
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG694620.12607750875402124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG211050.016.01915245
GGCATAG217200.015.67873335
ATAGGGG222200.015.45527238
GAGCTTA239350.014.26380544
CATAGGG249700.013.85195637
GGAGGTC353950.013.5189734
GGGAGGT369450.013.4323913
GCATAGG265100.013.05196236
TGGCATA266450.012.87341134
GTTGTCG28850.012.62299326
GGTCACT369100.012.4991817
GTCACTC377800.012.3845628
ACTCTCC386450.012.19571311
AGGTCAC429150.011.25316
GAGGTCA440150.011.110865
CACTCTC425400.011.02999110
CACCCGG137400.010.9625411
TAGGGGA423300.010.876492539
TCACTCT443950.010.7413639
TGGGAGG353450.010.6575062
Produced by FastQC (version 0.11.5)