FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53420974
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG544920.1020048792071818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG31250.022.175566
AACGCTA157000.019.2750823
ACGCTAC158100.019.11250924
GAACGAT178950.018.90991845
AGACCGC183000.018.4792294
CGCTACC169050.017.92775525
CAGACCG196450.017.3514823
TCACGCT184800.017.3007958
ACCGCGT196950.017.1702616
TCGACGA40650.017.0476327
GACCGCG198750.017.0147745
CGACGAG42250.016.5085498
CGGTATA59500.016.37362720
AAAACGC185300.016.34342821
CCGCGTT208650.016.1858777
CGCGTTC212100.015.92268
GAAAACG196550.015.45377720
AATACGG221300.015.23016932
AGAACGA223100.015.22828244
ATACGGA222400.015.19530833
Produced by FastQC (version 0.11.5)