FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50990700
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG30100.023.6897566
ACCGCGT104600.021.4833326
GACCGCG104950.021.3045625
AGACCGC110100.020.4512234
CCGCGTT116800.019.2586147
CGCGTTC118600.018.9852898
TCGACGA39550.018.0862467
CAGACCG130200.017.7086493
GTTCGAC39750.017.2596245
GAACGAT130750.016.91578345
CGGTATA54950.016.74283420
CGACGAG41550.016.5118858
AACGCTA114750.015.62378423
ACGCTAC115900.015.52750324
GTCCGTT39750.014.9511641
CGCTACC120650.014.91618425
ATACGGA147350.014.79879433
AATACGG150450.014.55292632
TCAGACC165250.014.5379212
CCCGTAT32150.014.2142281
Produced by FastQC (version 0.11.5)