FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57725027
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG165350.014.94748345
GGCATAG181750.013.7151135
GTTGTCG28400.013.38759926
ACCCGGC92300.013.0179765
ATAGGGG200600.012.63996138
CATAGGG201900.012.22411537
GAGCTTA207750.012.055344
GGGAGGT328250.011.6323723
TGGCATA217350.011.61360934
GCATAGG214500.011.46375336
TCACCCG109900.011.3833023
GGAGGTC317350.011.2592814
GGTCACT301000.011.1698647
GTCACTC312700.010.996628
ATAGCTC106200.010.97433939
ACGGACA111100.010.95514918
ACTCTCC320050.010.72298711
TGTCGTT37800.010.47502828
TAGGGGA319400.010.36921739
CGTCGAT29250.010.155745512
Produced by FastQC (version 0.11.5)