FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48186904
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2104210.43667673689930364No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1042320.21630773373612053No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG586590.1217322449269619No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA564440.11713556031738416No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT520310.10797747039320062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG41900.027.6428816
GAACGAT423250.024.1579745
TCGACGA50550.023.4020927
CGGTATA66900.023.36510320
ACCGCGT459650.022.5707656
GACCGCG461550.022.4778525
AGACCGC464700.022.4271184
CAGACCG470750.022.1866633
CGCGTTC470650.022.0384658
CCGCGTT472050.021.992167
CGACGAG54250.021.43298
GTTCGAC54250.021.3914435
TCAGACC506350.020.9465772
GCGTTCT515150.020.1871139
AGAACGA510350.020.07971444
AATACGG527950.019.45044532
ATACGGA532300.019.27572333
TACGGAG532150.019.13855434
CTCAGAC610350.018.8542521
CAATACG557250.018.5199231
Produced by FastQC (version 0.11.5)