FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48186904
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA710000.14734293782393657No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG645520.13396170876634864No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG579030.1201633539270338No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG485090.10066843057607519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTC289150.013.5419928
GGGAGGT313500.013.4517493
AGGTCAC307600.013.1107366
AGCTTAG237250.012.90921945
GGTCACT304850.012.8590847
GAGGTCA317450.012.8249535
AACTCTA108550.012.54219745
GGAGGTC328900.012.454914
GGCATAG250550.012.35636735
ACTCTCC318400.012.32889511
ATAGGGG261200.011.97100838
TATATAC49700.011.4941327
TGGCATA277400.011.24164434
GAGCTTA274550.011.22655544
ATATATA56850.011.0384196
TCACTCT358950.011.0354189
TCAGACG133900.011.0076662
TGGGAGG310150.010.6944922
CATAGGG299500.010.50760437
CACTCTC375450.010.40086710
Produced by FastQC (version 0.11.5)