FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52259056
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG130100.015.43523345
GGCATAG136500.014.60846635
ATAGGGG145700.013.90128138
CATAGGG145250.013.69743537
GAGCTTA158750.012.89013444
GCATAGG157600.012.63822436
GTCACTC216950.012.4947898
ACTCTCC227650.011.91058111
GGGAGGT256050.011.6146813
TGGCATA175300.011.555078534
GTTGTCG26800.011.33512426
TGAATCC159750.011.2765853
GGTCACT239700.011.2417337
AATCCAC149950.011.2401965
ATGAATC192150.011.04665852
CGTCGAT23450.010.74857212
AGGTCAC256600.010.7175436
CATGAAT231450.010.6614791
ACCCGGC83000.010.6407815
GGAGGTC271100.010.6053494
Produced by FastQC (version 0.11.5)