FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49555191
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG537860.1085375697573237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG181450.016.13104245
GGCATAG180650.016.07793835
ATAGGGG193950.015.09132738
GTCACTC247500.015.0100878
CATAGGG199150.014.71991537
GGTCACT257400.014.3986087
GAGCTTA204300.014.34884144
ACCCGGC83900.014.08414655
GTTGTCG31150.014.01121226
TGGCATA212700.013.81408734
ACTCTCC271200.013.79776211
GCATAGG216300.013.65681536
AGGTCAC290000.013.3780886
GGAGGTC289450.013.3362144
GAGGTCA309300.012.7929935
GGGAGGT334450.012.7878813
TCACTCT316550.011.9347099
TCACCCG103400.011.7992643
CACTCTC318400.011.68880910
CGGACAC102650.011.59508413
Produced by FastQC (version 0.11.5)