FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45372376
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1335350.29430903067540476No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG455180.10032095299571706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC269700.024.8166074
ACCGCGT273300.024.4803756
GACCGCG273950.024.4141065
TTCGACG25200.024.014226
CAGACCG282000.023.8559513
CCGCGTT287550.023.2749827
CGCGTTC296350.022.576258
GAACGAT296000.022.30159245
ATACGGA298850.021.88286433
AATACGG305000.021.83269732
TCAGACC312600.021.8086532
TACGGAG307600.021.20171534
GCGTTCT323100.020.7559329
CAATACG325800.020.47337331
AGAACGA335900.019.83351744
CGGTATA42400.019.47260920
CCCAATA346650.019.30668629
CTCAGAC391600.019.1172311
ACGGAGA342950.019.02233735
AAGAACG356000.018.63308143
Produced by FastQC (version 0.11.5)