FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75671135
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC799260.10562283755886574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC231950.018.064865
ACGGACA238450.017.42821318
TCACCCG251250.017.0970823
CGGACAC250650.016.56661613
CGGACAG253100.016.45480219
AGCTTAG209300.015.90018345
CACCCGG269200.015.8402954
CCGGACA275400.015.3151612
ATAGCTC267700.015.24152239
GGCATAG223450.014.93603735
ACACGGA281700.014.78107816
GCCCGGA287800.014.61020510
GACACGG288650.014.417191515
CACGGAC292700.014.38140117
CATAGGG244700.013.69394937
GTCACTC334650.013.4460278
GAGCTTA250000.013.41166144
TTGATAG310900.013.39124736
GGATTGA314300.013.3706225
TAGCTCT305200.013.33987140
Produced by FastQC (version 0.11.5)