FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41736669
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT34850.019.1951051
GTCGGTT39950.017.0829411
CTCGGTT94750.015.6892261
CCCGTAT31750.015.6778091
AGACGCT145050.015.07572741
CTCCGTT69900.014.7579181
GTCCGGT32950.014.6283441
ATGTCGC75650.014.54270835
ATGCGCG13800.014.50919344
AAGCGGC138300.014.48320328
CTCCGGT62750.014.1063811
CCGCAGA160850.013.93045937
CTCCGAT50050.013.9056471
CGGTTTT91550.013.7365773
CCCCGCA152550.013.67139635
TCGGTTT104350.013.6469732
GTCCGAT25100.013.6397581
CGTACCA90100.013.56345330
CCCGGAT89050.013.4053681
CTCGCGT6900.013.3835581
Produced by FastQC (version 0.11.5)