FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41736669
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG103300.015.48352445
ATAGGGG105850.015.43030238
GGCATAG108600.014.68807335
CATAGGG115850.013.92360737
GAGCTTA124150.013.0825444
GCATAGG124550.012.861217536
TGGCATA134950.012.07024834
GGGAGGT191250.011.3064713
GGAGGTC195450.010.3612344
TAGGGGA190200.010.27832439
ACTCTCC201500.010.07334111
TAGCGTA6150.09.87682337
TCGCGCT27450.09.67030645
TGCCCGT96500.09.06825744
AGGGGAG236650.08.5554840
TAATCGG24050.08.5139474
GGGGAGC239400.08.49478941
CGTAGGG51650.08.40611540
TCTCCCC244150.08.40583213
GCGTAGG52150.08.36874839
Produced by FastQC (version 0.11.5)