FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49176149
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA138800.020.8211323
ACGCTAC139700.020.68703524
CGCTACC146400.019.72497725
AAAACGC159000.018.28780721
TCACGCT167400.018.2376148
GAAAACG172950.016.8639120
ACGCTCA192300.016.43562310
GCCGGCT195450.016.3500791
CCGGCTT205850.016.2805772
CCCGTAT30900.016.0388971
TTCACGC191950.015.9868067
AAACGCT183800.015.96715322
CACGCTC194900.015.4918089
ACGGTAC73600.015.45517945
GTTTTCG188700.015.35457545
CGGCTTC237300.014.7650683
TACGGTA77350.014.72245544
TCGTGGT201350.014.39446440
CGTGGTT206100.014.13959241
TTCGACG18750.014.1067546
Produced by FastQC (version 0.11.5)