FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49176149
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG125700.013.44443745
GGCATAG137950.012.70833235
ATAGGGG149350.012.11099838
CATAGGG152700.011.55390537
GAGCTTA152900.011.26302844
GTCACTC207150.011.1153168
GCATAGG160900.010.93715836
GGTCACT207200.010.8549947
GGGAGGT247300.010.5524553
CACCGAA142150.010.49438245
GCACCGA142850.010.461781544
AGGTCAC231850.010.2573096
ACCCGGC80950.010.1956435
GGAGGTC230900.010.1071164
TGGCATA181550.09.92593634
ACTCTCC233750.09.85035811
CATTCGG48600.09.5214424
GAGGTCA251250.09.491755
TCGCGCT39600.09.3332445
TAGGGGA240700.09.28009239
Produced by FastQC (version 0.11.5)