FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48118743
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT909650.18904276032314477No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT497600.10341084761919073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC190100.025.166044
ACCGCGT194000.024.5094036
GACCGCG197150.024.1862535
CAGACCG203550.023.6688883
CCGCGTT205350.023.1656867
CGCGTTC212400.022.4071488
TCAGACC241700.020.9569452
TTCGACG22800.020.3217856
GCGTTCT254000.018.8701429
GAACGAT258300.018.5393445
CTCAGAC339650.017.94871
TTCGGCG117250.017.62331244
ATACGGA267550.017.53997633
AATACGG271000.017.53205732
TACGGAG269400.017.30299434
GTTAGTT152100.017.18148444
AACGCTA186100.017.06974423
TCGGCGC120750.017.01230245
ACGCTAC187500.016.94229124
AGAACGA291450.016.53523344
Produced by FastQC (version 0.11.5)