FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46534626
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG116900.014.14585335
CATAGGG121150.013.62900237
ATAGGGG125250.013.54141438
AGCTTAG125650.013.2103545
ACCCGGC87700.012.858845
GTCACTC196600.012.24700458
GCATAGG133950.012.24368636
GGTCACT198400.012.0111837
GAGCTTA142950.011.84762544
ACTCTCC210000.011.7971511
TGGCATA147300.011.334022534
TAGGGGA211900.011.146070539
GGCGTAG64250.011.0654835
GTTGTCG23200.011.05745926
GCGTAGG63900.011.054934536
AGGTCAC226050.011.0203086
ATAGCTC91300.011.0128639
CGTAGGG64150.011.0109437
ACCGGTA34400.010.91889145
GGGAGGT258250.010.8364483
Produced by FastQC (version 0.11.5)