FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52898679
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG817430.15452748829512358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA195050.022.24115823
ACGCTAC198500.021.8435124
CGCTACC201450.021.46778925
GCCGGCT249700.020.0541111
CCGGCTT242350.019.7917142
AAAACGC221600.019.63616821
ACGCTCA232750.019.3696510
TTCACGC231600.019.3394287
GAAAACG231450.018.85869820
TCACGCT237300.018.7990868
CACGCTC242850.018.404659
AAACGCT243100.017.98309522
CGGCTTC282850.017.482793
CGTGGTT258950.016.83022341
GTTTTCG261650.016.53954345
CTACCTC277700.015.84061827
TCGTGGT275050.015.80524440
ACGTTCG17000.015.3502646
CCCGTAT31050.015.1487461
TACCTCT293900.014.99809528
Produced by FastQC (version 0.11.5)