FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48687428
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT922600.18949450359135833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG41900.026.0959916
TCGACGA45600.023.9785797
GAACGAT190200.022.29090345
ACCGCGT192400.022.018966
AGACCGC194450.021.741374
CGGTATA67600.021.50016820
CGACGAG50750.021.1020958
GACCGCG203650.020.7477385
CCGCGTT204650.020.689977
CAGACCG206650.020.5775663
CGCGTTC209200.020.2400998
TCGGCGC62050.018.93154145
AGAACGA232500.018.27413244
TCAGACC237900.018.1959652
ATACGGA244150.017.38014633
TACGGAG244550.017.37980334
AATACGG245700.017.21499632
GCGTTCT252550.016.8995659
TTCGGCG69150.016.89012544
ATGACGG80100.016.48784316
Produced by FastQC (version 0.11.5)