FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48687428
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG29500.015.55728826
AGCTTAG108750.014.40357745
ACCCGGC83750.013.7850035
GGCATAG114900.013.49248235
TGTCGTT37300.012.84668628
ACGGACA90450.012.33792418
ATAGGGG130150.012.27481238
GCTAAGT42500.011.75292614
ATAGCTC93850.011.60476339
GAGCTTA137250.011.57665644
TCACCCG102800.011.5149263
GTCGTTC42000.011.35537729
AACTCTA114050.011.14919545
ATATAGC51850.010.9786356
TGGCATA143300.010.97521234
CATAGGG146150.010.90010437
CCGGACA105050.010.70899612
CGGACAC104750.010.69668613
GGGAGGT276950.010.5960673
TATAGCT52800.010.5251117
Produced by FastQC (version 0.11.5)