FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51113578
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCT954910.1868212004254525No Hit
CAGGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATT576620.11281151164960511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCTC162150.018.6128379
GCGCTCT162950.018.2998510
GCAGCGC167950.017.6354437
CGCTCTC171350.017.61424811
CAGCGCT170750.017.5175178
CGGGACG173000.016.8385817
TCTCGGG181100.016.54354314
GACCTCC360850.016.2240145
TCGGGAC182400.016.15463816
GTTGTCG30400.015.97364826
GCTCTCG187700.015.91368412
TGTGCAA350950.015.88241212
ACGTCTC186500.015.77696821
GTGCAAG358850.015.62695713
CTCTCGG203850.015.06110413
AGCTTAG164200.014.86219845
GGACCTC409600.014.71622854
GGGACGT198200.014.70902118
CTCGGGA206250.014.47174615
TGGACCT417650.014.222783
Produced by FastQC (version 0.11.5)