FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42294051
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG754810.1784671796986295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTC301050.015.9363958
AGCTTAG223750.015.79694945
GGTCACT302400.015.7690577
ACGGACA109900.015.580152518
ACCCGGC113000.015.4939735
AGGTCAC322800.014.98864656
GAGGTCA330650.014.8656545
TCACCCG121950.014.8551673
ACTCTCC328400.014.6502111
GGCATAG240050.014.628509535
TAGCGTA15050.014.3658837
CGGACAG119350.014.13941519
CGGACAC125250.014.13771713
GAGCTTA258100.013.80786844
ATAGCTC118250.013.76940239
GGGAGGT384250.013.6524723
TGGCATA261150.013.58464234
TCACTCT362500.013.5200569
CCGGACA134350.013.34738212
GGAGGTC368950.013.2741074
Produced by FastQC (version 0.11.5)