FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56649199
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG951070.16788763420997357No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN695370.12275019104859718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG288000.017.0339445
GGCATAG323750.015.07567135
GAGCTTA334450.014.78857944
GTCACTC451600.014.7233288
GGGAGGT478350.014.6540523
ATAGGGG338300.014.638988538
GGTCACT461800.014.460877
GGAGGTC495400.013.855354
AGGTCAC493100.013.8028346
TGGCATA359950.013.68562634
ACTCTCC497050.013.62701411
GAGGTCA509650.013.496115
CATAGGG386550.012.85890237
TCACTCT539200.012.7071289
CACTCTC539450.012.58908810
TGGGAGG420550.012.3578152
GCATAGG405550.012.26233636
CACCCGG169650.012.1166361
ACCCGGG168850.011.7070432
TGCCCGT179350.011.36728544
Produced by FastQC (version 0.11.5)