FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49243655
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3943150.8007427555895272No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG987390.2005111115330493No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG945400.19198412465524747No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA933610.18958990757286395No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC676430.1373638898249937No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC661490.1343299964228894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT643350.029.48731445
AGAACGA752850.025.20960844
GACCGCG802450.023.755875
AGACCGC805400.023.752824
ACCGCGT806050.023.6801366
CAGACCG814150.023.5280763
CCGCGTT818200.023.3257437
CGCGTTC824200.023.1668538
TCAGACC847150.022.842592
AATACGG856900.022.08537932
ATACGGA858250.022.03846733
TACGGAG857950.021.9597834
AAGAACG865950.021.89046343
GCGTTCT879000.021.755939
CAATACG885250.021.47189931
CTCAGAC937750.021.3844661
ACGGAGA893800.021.10220335
CCCAATA914800.020.893429
CACTCCC937850.020.50220924
TCGGCGC168350.020.46767645
Produced by FastQC (version 0.11.5)