FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47493953
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1055720.22228514017352904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT190500.026.62011145
AGACCGC197450.025.977954
ACCGCGT197650.025.9175726
GACCGCG198950.025.782145
CAGACCG206350.025.0537223
CCGCGTT210800.024.332717
TTCGACG35200.024.2867286
CGCGTTC212300.024.1607868
ATACGGA223200.022.77716433
AATACGG224950.022.6520932
TACGGAG225500.022.28466234
CGGTATA56000.022.01523620
AGAACGA232950.021.97123744
TCAGACC241150.021.7740352
TCGACGA40050.021.626417
CAATACG247500.020.67075231
AACGCTA112850.020.63581823
GCGTTCT254850.020.215079
ACGCTAC115900.020.13276324
AAGAACG255100.020.04605543
Produced by FastQC (version 0.11.5)