FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47493953
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG121300.015.16779845
GGCATAG132000.013.92531635
ATAGGGG139750.013.40650938
CATAGGG141000.013.08312437
GAGCTTA145900.012.95040344
GCATAGG151050.012.33217836
GTCACTC211200.011.631468
GGTCACT209000.011.6139937
TGGCATA166250.011.21888334
GTTGTCG27250.011.06805826
ACTCTCC231350.010.94901811
ATAGCTC80300.010.52895939
GGGAGGT258200.010.5219013
AGGTCAC245850.010.3932276
TAGGGGA238150.010.04464439
ACCCGGC89850.010.0205565
GAGGTCA265550.09.8163825
TAGCGTA12250.09.75701637
AACTCTA107700.09.60926545
GGAGGTC266400.09.5912744
Produced by FastQC (version 0.11.5)