FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53374860
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG596120.11168553884731501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG207450.015.37805145
GGCATAG220150.014.10956335
GGGAGGT319350.013.6317693
ATAGGGG235000.013.61512838
GGAGGTC312450.013.2764844
GTCACTC310850.013.0353128
GTTGTCG31500.013.0094526
GAGCTTA245550.012.92327944
GGTCACT314550.012.7034897
TGGCATA251650.012.52232834
CATAGGG254800.012.31957637
ACTCTCC334500.012.07297511
GCATAGG268600.011.75364736
CACCGAA297800.011.70144245
GCACCGA309500.011.21220144
AGGGATC139500.011.1623632
GTTCTAT233500.011.0728291
GAGGTCA387500.011.0561215
AGGTCAC389400.010.7703396
TGTCGTT38100.010.75708828
Produced by FastQC (version 0.11.5)