FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44480516
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG901100.20258308154518712No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT685490.1541101726427814No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA616980.138707923262401No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG448520.10083516117483889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC141150.023.7277974
CAGACCG153600.022.1568973
GACCGCG157850.021.2603655
ACCGCGT170300.019.7720956
CTACACG49550.016.97970245
TCAGACC212550.016.2554862
CTCAGAC244100.016.2130071
TTCGACG13850.015.10353856
ACGCTAC167050.015.04485324
AACGCTA169350.014.800123
CGCTACC174800.014.31387825
TCGGCGC67600.013.67720245
CCGCGTT247000.013.61414057
CCCGTAT25000.013.4247391
CGTACGC68950.013.4076935
TTCGGCG68850.013.39735844
CGTGGTT188400.013.37556341
AAAACGC190100.013.37280221
ACGTTCG16150.013.3703956
GCACTAC69250.013.28915825
Produced by FastQC (version 0.11.5)