FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44480516
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA664680.14943171972195646No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG647980.14567726687343285No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG530120.11918027209936145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG171000.015.47395645
GTTGTCG25100.015.34104126
GGCATAG179100.014.89500635
ATAGGGG182600.014.75431638
GAGCTTA188650.014.08748444
GTCACTC257050.013.8042398
CATAGGG200600.013.39712137
GGTCACT264200.013.3376787
GGGAGGT308200.012.9508043
AGGTCAC280400.012.8880936
TGGCATA207700.012.85601834
GGAGGTC285300.012.7067464
ACTCTCC280350.012.6327611
GCATAGG216000.012.51676136
ACCCGGC76100.012.3667525
TCACTCT298900.012.1118419
TAGCGTA9600.011.9774737
GAGGTCA303100.011.9232975
ATATAGC38850.011.8515046
CACTCTC302650.011.679710
Produced by FastQC (version 0.11.5)