FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42240690
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA967640.22907769735768993No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG806370.19089887025993182No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT707100.16739783370015973No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG618890.1465151255815187No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG467650.1107107862111154No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG426760.10103054661275655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA108350.017.24511745
TATATAC49650.016.0129417
ATATATA47800.014.1899746
CCATATA49800.013.8914014
CATGCAT74150.013.5987879
TCAGACG131400.013.2305062
AGCTTAG171350.012.8645745
GTCACTC226200.012.6784268
GGCATAG177050.012.58372235
ATAGGGG181100.012.49869738
AGGGATC156800.012.3478712
GGTCACT230050.012.2520467
ATATAGC32800.012.1884576
GGAGGTC246000.011.9425564
GGGAGGT259050.011.8440573
CATATAT58150.011.74206355
ATATACA73700.011.7316828
ATACATG87400.011.6666716
GTTGTCG32050.011.66315426
TTCCATA77200.011.6378712
Produced by FastQC (version 0.11.5)