FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46892688
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG719660.1534695558505838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG214300.018.39033945
GGCATAG232700.016.91341635
GTTGTCG36300.016.8016826
ATAGGGG241350.016.22177138
GAGCTTA250600.015.86124644
TGGCATA257700.015.38691834
GGTCACT372250.014.91115957
GGGAGGT401300.014.7463383
GTCACTC384650.014.6172658
GGAGGTC388500.014.6015014
CATAGGG280050.014.10888137
AGGTCAC405250.014.0463266
GAGGTCA413250.013.9007855
ACTCTCC413450.013.74008211
TGTCGTT45550.013.61212128
ATATAGC53800.013.575376
GCATAGG300400.013.13064836
TGGGAGG323350.012.9491992
TAGGGGA427200.012.84097139
TCACTCT452600.012.6610639
Produced by FastQC (version 0.11.5)