FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46119142
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1222180.2650049300570249No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG553390.11999139099335368No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG497480.10786844213190262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT488550.016.7982393
GTCACTC476200.016.6682938
AGGTCAC488650.016.4989936
GGTCACT481900.016.4253837
GGAGGTC500200.016.1865314
GAGGTCA504000.016.1490145
ACTCTCC532250.014.99749411
AGCTTAG402800.014.98029845
TGGGAGG422350.014.9601512
TCACTCT535300.014.9002559
CACTCTC556500.014.24403910
GGCATAG445750.013.57618335
ATAGGGG457100.013.29890438
CTGGGAG516700.013.11796951
GAGCTTA464400.013.09086344
TGGCATA472750.012.85847334
TGCCCGT206950.012.4593244
TCTCCCC655100.012.29104813
AGGCTCT512700.012.16331120
GCTCTGT678700.011.74758522
Produced by FastQC (version 0.11.5)