FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49298095
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG764770.15513175509114502No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT715690.14517599513733745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC152250.024.3074324
ACCGCGT157200.023.6534676
TTCGACG24950.023.621386
GACCGCG160750.023.0914655
CCGCGTT171600.021.6815387
CAGACCG175700.021.1027153
CGCGTTC173850.021.077488
CGGTATA40400.019.3211420
GAACGAT187500.019.24317445
AACGCTA254950.018.93719923
ACGCTAC254900.018.92456624
TCGACGA32650.018.8772747
TCACGCT264300.018.775038
TCAGACC203450.018.556122
TTCACGC273900.018.437257
CGCTACC267700.018.01236525
AAAACGC275700.017.59174721
AATACGG206950.017.47057232
GCGTTCT212150.017.3571159
ATACGGA210400.017.248333
Produced by FastQC (version 0.11.5)