FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53812913
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1362660.2532217499543279No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN660520.12274377341364145No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA584250.10857059531417673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT542200.016.8101143
GTTGTCG45100.016.11623426
GGAGGTC564300.015.8059754
TGGGAGG473200.015.6303772
GTCACTC556100.015.6047038
AGCTTAG454550.015.4361945
GGTCACT564950.015.3836837
AGGTCAC580750.015.3251386
GAGGTCA582850.015.2779545
ACTCTCC589800.014.80561511
ATATAGC54650.014.7339936
TCACTCT616650.014.2041869
GGCATAG504400.013.95049335
CTGGGAG575850.013.9423321
ATAGGGG519750.013.68460838
CACTCTC634650.013.64224110
GAGCTTA527050.013.41468844
CACCCGG145300.013.3264391
TGGCATA535050.013.21095534
TGTCGTT58600.012.90311528
Produced by FastQC (version 0.11.5)