FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50193262
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT775560.15451476335608552No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT679100.13529704445190271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG37150.028.8892976
TCGACGA41850.025.8061247
ACCGCGT164150.025.2479526
AGACCGC168850.024.9185224
GACCGCG169700.024.3294125
CGGTATA60700.023.7970620
CAGACCG178650.023.6775383
CCGCGTT181200.022.847397
CGCGTTC184650.022.4570668
CGACGAG48700.021.8991058
TCAGACC216850.020.6998372
AACGCTA216700.020.01820423
ACGCTAC217750.019.94233924
GAACGAT220350.019.1149945
TCACGCT237650.019.0677138
AATACGG218650.018.95491632
CCGGTTT110850.018.6942212
AAAACGC233450.018.63972721
CGCTACC232350.018.6117725
TGCGCGT63400.018.59616345
Produced by FastQC (version 0.11.5)