FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54550678
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG160800.015.00619645
GGCATAG173200.013.89984835
ATAGGGG182750.013.41155338
CATAGGG188650.012.81036537
GAGCTTA193300.012.78639144
GCATAGG192650.012.52104536
GTTGTCG33650.012.49514526
GTCACTC250700.012.3239848
CGTCGAT31450.012.1497812
GGGAGGT284800.011.7423143
TGGCATA213450.011.54832434
TGCGTCG34600.011.23532110
GCGTCGA35000.011.10880211
ACTCTCC280800.011.10116511
GGAGGTC294400.010.9395424
AGGTCAC294450.010.8660516
GAGGTCA304850.010.733285
GGTCACT293900.010.5882037
TCAGACG86050.09.9769112
TCACTCT320750.09.8987749
Produced by FastQC (version 0.11.5)